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anti rrm2  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology anti rrm2
    Anti Rrm2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+rrm2/R2+Antibody/pm41813908-121-36-39
    Average 93 stars, based on 108 article reviews
    anti rrm2 - by Bioz Stars, 2026-09
    93/100 stars

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    other:

    Article Title: mTORC2 regulates ribonucleotide reductase to promote DNA replication and gemcitabine resistance in non-small cell lung cancer.
    Article Snippet: Anti-RRM1(sc377415), anti-RRM2(sc-398294) anti- β-Actin(sc-47778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). p-Akt substrate (RXXS/T) (#9614), BrdU (#5292), Ki67(#9027), Cleaved caspase-3 (#9579) and pAkt S473 (#4060) antibodies were purchased from Cell Signaling Technology (Danvers, MA). γ H2AX (05-636) was purchased from Millipore (Billerica, MA).

    Article Title: Pleiotropic Effects of Metformin on the Chemotherapy Response of HPV-Positive Cancer Cells.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Cancer Research Center, Heidelberg, Germany); anti‐B‐MYB (LX015.1, from Dr. Roger Watson) [43]; anti‐BID (#2002), anti‐ cleaved Caspase‐9 (#7237), anti‐cleaved poly(ADP‐ribose) polymerase (PARP) (#9546), anti‐FOXM1 (D3F2B, #20549), anti‐ acetyl‐p53 (K382) (#2525), anti‐phospho‐p53 (S15) (#9284), anti‐ p130 (D9T7M, #13610), anti‐pRb (#9309), anti‐phospho‐pRb (S807/811) (#9308), all from Cell Signaling Technology; anti‐ CKS1 (36‐6800), from Invitrogen, Thermo Fisher Scientific; anti‐β‐ Actin (C4, sc‐47778), anti‐CDC2 (B‐6, sc‐8395), anti‐CDK2 (D‐12, sc‐6248), anti‐Cyclin A (H‐432, sc‐751), anti‐Cyclin B2 (A‐2, sc‐ 28303), anti‐Cyclin D1 (DCS‐6, sc‐20044), anti‐E2F1 (KH95, sc‐ 251), anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (FL‐335, sc‐25778), anti‐p21 (SX118, sc‐53870), anti‐p53 (DO‐1, sc‐ 126), anti‐PCNA (PC10, sc‐56), anti‐PLK1 (F‐8, sc‐17783), anti‐ RRM1 (R1 [A‐10], sc‐377415), anti‐RRM2 (R2 [A‐5], sc‐398294), all from Santa Cruz Biotechnology; anti‐Cyclin B1 (05‐373, clone GNS3 [8A5D12]), from Upstate; anti‐E6AP (E8655, clone E6AP‐ 300), from Sigma‐Aldrich.

    Article Title: mTORC2 regulates ribonucleotide reductase to promote DNA replication and gemcitabine resistance in non-small cell lung cancer
    Article Snippet: Anti-RRM1(sc-377415), anti-RRM2(sc-398294) anti-β-Actin(sc-47778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). p-Akt substrate (RXXS/T) (#9614), BrdU (#5292), Ki67(#9027), Cleaved caspase-3 (#9579) and pAkt S473 (#4060) antibodies were purchased from Cell Signaling Technology (Danvers, MA). γH2AX (05-636) was purchased from Millipore (Billerica, MA).

    Article Title: The scaffold protein IQGAP1 links heat-induced stress signals to alternative splicing regulation in gastric cancer cells
    Article Snippet: Protein A/G Plus Agarose Beads were purchased from Santa-Cruz Biotechnology (sc-2003) The following antibodies were used: anti-hnRNPM, clone 1D8 (Santa Cruz Biotechnology, Cat# sc-20002; or NB200-314SS, Novus); anti-IQGAP1 (clone H109 Santa Cruz Biotechnology, Cat# sc-10792; RRID:AB_2249072; clone D-3, Santa Cruz, Cat# sc-374307; clone C-9, Santa Cruz, Cat# sc-379021; Proteintech, Cat# 22167-1-AP); anti-Beta-actin (clone 7D2C10, ProteinTech, Cat# 60008-1-Ig); anti-Lamin B1 (clone A-11, Santa Cruz Cat#sc-377000); anti-GAPDH (ProteinTech, Cat#60004-1-Ig); anti-hnRNP A2/B1 (clone DP3B3, Santa Cruz Cat#sc-32316); anti-hnRNP A1 (clone 4B10, Santa-Cruz Biotechnology, Cat# sc-32301); anti-hnRNPL (clone 4D11, Santa-Cruz Biotechnology Cat# sc-46673); anti-hnRNP C1/C2 (clone 4F4, Santa-Cruz Biotechnology, Cat#sc-32308); anti-RNA Helicase A (Abcam Cat# ab26271); anti-hnRNP K/J (clone 3C2, Santa-Cruz Biotechnology Cat# sc-32307); anti-SF3B3 (clone B-4, Santa Cruz, Cat# sc-398670); anti-beta tubulin (clone 2-28-33, Sigma-Aldrich, Cat# T5293); anti-TPX2 (clone E-2, Santa-Cruz Biotechnology, Cat# sc-271570); anti-RRM2 (clone A-15, Santa-Cruz Biotechnology, Cat# sc-398294); anti-SAFB (F-3, Santa Cruz, Cat# sc-393403); anti-Matrin3 (Santa-Cruz Biotechnology, Cat#2539a); anti-Histone H3 (ProteinTech, Cat#17168-1-AP); anti-CPSF6 (clone H-59, Santa-Cruz Biotechnology, Cat#sc-292170); anti-NF90 (clone A-3, Santa-Cruz Biotechnology, Cat#sc-377406); anti-Anillin (CL0303, Abcam, Cat# ab211872); anti-FZR, (clone DSC-266, Santa-Cruz Biotechnology, Cat# sc-56312); anti-TK1 (EPR3193, Abcam, Cat# ab76495); anti-ANAPC10 (clone B-1, Santa-Cruz Biotechnology, Cat# sc-166790); anti Ki67 (clone SolA15, ThermoFisher, Cat# 14-5698-82); HRP-conjugated goat anti-rabbit (SouthernBiotech, Cat# 4050-05); HRP-conjugated goat anti-mouse IgG (SouthernBiotech, Cat# 1030-05); Anti-rabbit-Alexa Fluor 555 (Molecular Probes, Cat# A27039); Anti-mouse Alexa Fluor 488 (Molecular Probes Cat# A28175); Anti-mouse Alexa Fluor 647 (Invitrogen, Cat#A21235).

    Article Title: Pleiotropic Effects of Metformin on the Chemotherapy Response of HPV‐Positive Cancer Cells
    Article Snippet: The following primary antibodies were used: anti‐HPV18 E6 (AVC 399) and anti‐HPV16 E6 (AVC 843, kind gifts from Dr. Johannes Schweizer, Arbor Vita Corporation, Fremont, CA, USA); anti‐HPV18 E7 (E7C) [ ]; anti‐HPV16 E7 (NM2, kind gift from Dr. Martin Müller, German Cancer Research Center, Heidelberg, Germany); anti‐B‐MYB (LX015.1, from Dr. Roger Watson) [ ]; anti‐BID (#2002), anti‐cleaved Caspase‐9 (#7237), anti‐cleaved poly(ADP‐ribose) polymerase (PARP) (#9546), anti‐FOXM1 (D3F2B, #20549), anti‐acetyl‐p53 (K382) (#2525), anti‐phospho‐p53 (S15) (#9284), anti‐p130 (D9T7M, #13610), anti‐pRb (#9309), anti‐phospho‐pRb (S807/811) (#9308), all from Cell Signaling Technology; anti‐CKS1 (36‐6800), from Invitrogen, Thermo Fisher Scientific; anti‐β‐Actin (C4, sc‐47778), anti‐CDC2 (B‐6, sc‐8395), anti‐CDK2 (D‐12, sc‐6248), anti‐Cyclin A (H‐432, sc‐751), anti‐Cyclin B2 (A‐2, sc‐28303), anti‐Cyclin D1 (DCS‐6, sc‐20044), anti‐E2F1 (KH95, sc‐251), anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (FL‐335, sc‐25778), anti‐p21 (SX118, sc‐53870), anti‐p53 (DO‐1, sc‐126), anti‐PCNA (PC10, sc‐56), anti‐PLK1 (F‐8, sc‐17783), anti‐RRM1 (R1 [A‐10], sc‐377415), anti‐RRM2 (R2 [A‐5], sc‐398294), all from Santa Cruz Biotechnology; anti‐Cyclin B1 (05‐373, clone GNS3 [8A5D12]), from Upstate; anti‐E6AP (E8655, clone E6AP‐300), from Sigma‐Aldrich.

    Western Blot:

    Article Title: Osteoblast-derived osteomodulin restrains osteoclastogenesis via ITGB8/RRM2-mediated reduction of mitochondrial respiration and mitochondrial ATP production
    Article Snippet: Protein bands were visualized using the Amersham Imager 600 (GE) and quantified using ImageJ. .. Western blotting (WB) was performed with the following antibodies: anti-GAPDH (1:10,000, 60004-1-IG; Proteintech), anti-β-actin (1:10,000, 66009-1-IG; Proteintech), anti-cathepsin K (CTSK) (1:100, sc-48353; Santa Cruz), anti-NFATC1 (1:100, sc-7294; Santa Cruz), anti-matrix metallopeptidase 9 (MMP9) (1:1,000, 10375-2-AP; Proteintech), anti-RRM2 (1:100, sc-81850; Santa Cruz), anti-ITGB8 (1:1,000, 29775-1-AP; Proteintech), anti-OMD (1:1,000, ab154249; Abcam), anti-RUNX2 (1:1,000, 20700-1-AP; Proteintech), anti-collagen type I (1:1,000, 14695-1-AP; Proteintech), anti-ATP Synthase F1 Subunit Alpha (ATP5A1) (1:10,000, 14676-1-AP; Proteintech), anti-ubiquinol-cytochrome c reductase core protein 2 (UQCRC2) (1:10,000, 67547-1-Ig; Proteintech), anti-succinate dehydrogenase subunits B (SDHB) (1:10,000, 10620-1-AP; Proteintech), anti-cytochrome oxidase subunit IV (MTCO4) (1:1,000, 11242-1-AP; Proteintech), anti-NADH-ubiquinone oxidoreductase ASHI subunit (NDUFB8) (1:10,000, 14794-1-AP; Proteintech), anti-FLAG (1:10,000, 20543-1-AP; Proteintech), anti-HA (1:10,000, 51064-2-AP; Proteintech), anti-YAP (1:1,000, ET1608-30; HUABIO), anti-p-YAP (S127) (1:1,000, ET1611–69; HUABIO), anti-Smad2 (1:1,000, ET1604-22; HUABIO), anti-p-Smad2 (1:5,000, ET1612-32; HUABIO), anti-Smad3 (1:5,000, ET1607-41; HUABIO), anti-p-Smad3 (1:2,000, ET1609-41; HUABIO), anti-AKT (1:1,000, #9272, Cell Signaling Technology), anti-p-AKT (1:2,000, #4060, Cell Signaling Technology), anti-ERK (1:1,000, #9102, Cell Signaling Technology), anti-p-ERK (1:1,000, #4695, Cell Signaling Technology), anti-p38 (1:1,000, #9212, Cell Signaling Technology), anti-p-p38 (1:1,000, #4511, Cell Signaling Technology), anti-JNK (1:5,000, 51153-1-AP; Proteintech) and anti-p-JNK (1:1,000, 80024-1-RR; Proteintech). ..

    Article Title: Osteoblast-derived osteomodulin restrains osteoclastogenesis via ITGB8/RRM2-mediated reduction of mitochondrial respiration and mitochondrial ATP production.
    Article Snippet: Protein bands were visualized using the Amersham Imager 600 (GE) and quantified using ImageJ. .. Western blotting (WB) was performed with the following antibodies: anti-GAPDH (1:10,000, 60004-1-IG; Proteintech), anti-β-actin (1:10,000, 66009-1-IG; Proteintech), anti-cathepsin K (CTSK) (1:100, sc-48353; Santa Cruz), anti-NFATC1 (1:100, sc-7294; Santa Cruz), anti-matrix metallopeptidase 9 (MMP9) (1:1,000, 10375-2-AP; Proteintech), anti-RRM2 (1:100, sc-81850; Santa Cruz), anti-ITGB8 (1:1,000, 29775-1-AP; Proteintech), anti-OMD (1:1,000, ab154249; Abcam), antiRUNX2 (1:1,000, 20700-1-AP; Proteintech), anti-collagen type I (1:1,000, 14695-1-AP; Proteintech), anti-ATP Synthase F1 Subunit Alpha (ATP5A1) (1:10,000, 14676-1-AP; Proteintech), anti-ubiquinol-cytochrome c reductase core protein 2 (UQCRC2) (1:10,000, 67547-1-Ig; Proteintech), anti-succinate dehydrogenase subunits B (SDHB) (1:10,000, 10620-1-AP; Proteintech), anti-cytochrome oxidase subunit IV (MTCO4) (1:1,000, 11242-1-AP; Proteintech), anti-NADH-ubiquinone oxidoreductase ASHI subunit (NDUFB8) (1:10,000, 14794-1-AP; Proteintech), anti-FLAG (1:10,000, 20543- 1-AP; Proteintech), anti-HA (1:10,000, 51064-2-AP; Proteintech), anti-YAP (1:1,000, ET1608-30; HUABIO), anti-p-YAP (S127) (1:1,000, ET1611–69; HUABIO), anti-Smad2 (1:1,000, ET1604-22; HUABIO), anti-p-Smad2 (1:5,000, ET1612-32; HUABIO), anti-Smad3 (1:5,000, ET1607-41; HUABIO), anti-p-Smad3 (1:2,000, ET1609-41; HUABIO), anti-AKT (1:1,000, #9272, Cell Signaling Technology), anti-p-AKT (1:2,000, #4060, Cell Signaling Technology), anti-ERK (1:1,000, #9102, Cell Signaling Technology), anti-p-ERK (1:1,000, #4695, Cell Signaling Technology), anti-p38 (1:1,000, #9212, Cell Signaling Technology), anti-p-p38 (1:1,000, #4511, Cell Signaling Technology), anti-JNK (1:5,000, 51153-1-AP; Proteintech) and anti-p-JNK (1:1,000, 80024-1-RR; Proteintech). ..

    Article Title: MS CETSA deep functional proteomics uncovers DNA repair programs leading to gemcitabine resistance
    Article Snippet: .. For protein detection with western blot, the following antibodies were used: Santa Cruz Biotechnology: anti-RRM2 (sc-81850), anti-GMNN (sc-74456), anti-PCNA (sc-56), anti-PARP1 (sc-8007), anti-PolK (sc-166667), anti-PolH (sc-17770), anti-PolI (sc-101026), anti-Rev1 (sc-393022), anti-beta actin (sc-69879), anti-goat IgG HRPconjugated secondary antibody (sc-2354). .. Invitrogen: anti-SLBP (PA5-53966), anti-FBXO5 (37-6600), anti-DTL (PA5-88380), antirabbit IgG HRP-conjugated secondary antibody (31460), anti-mouse IgG HRP-conjugated secondary antibody (31430).



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    Expression of several senescence-associated proteins under HS conditions at 41 °C and 45 °C for 30 min in aged HFF-1 cells. (a) Detection of protein expression levels of GORAB, AURKB, RhoC, α-N-catenin, RRM2, AURKA, and PLK1 under 41 °C and 45 °C HS. Protein expression was quantified as fold-change relative to untreated controls (set to 1), normalized to GAPDH. (b) GORAB. (c) AURKB. (d) RhoC. (e) α-N-catenin. (f) RRM2. (g) AURKA. (h) PLK1. Representative blots were cropped only to improve presentation; the corresponding full-length, uncropped blots with molecular-weight markers and the associated GAPDH loading control are provided in <xref ref-type=Supplementary Figure S1 (the first lane corresponds to an unrelated sample and was not analyzed). Data are representative of three independent experiments, with results presented as mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001, compared with the control group. " width="100%" height="100%">

    Journal: Cell Stress & Chaperones

    Article Title: Proteomic and phenotypic profiling of replicative-senescent human foreskin fibroblasts under brief heat shock

    doi: 10.1016/j.cstres.2026.100174

    Figure Lengend Snippet: Expression of several senescence-associated proteins under HS conditions at 41 °C and 45 °C for 30 min in aged HFF-1 cells. (a) Detection of protein expression levels of GORAB, AURKB, RhoC, α-N-catenin, RRM2, AURKA, and PLK1 under 41 °C and 45 °C HS. Protein expression was quantified as fold-change relative to untreated controls (set to 1), normalized to GAPDH. (b) GORAB. (c) AURKB. (d) RhoC. (e) α-N-catenin. (f) RRM2. (g) AURKA. (h) PLK1. Representative blots were cropped only to improve presentation; the corresponding full-length, uncropped blots with molecular-weight markers and the associated GAPDH loading control are provided in Supplementary Figure S1 (the first lane corresponds to an unrelated sample and was not analyzed). Data are representative of three independent experiments, with results presented as mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001, compared with the control group.

    Article Snippet: Membranes were blocked with 5% nonfat milk for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against GORAB (Proteintech, Wuhan, China; 17798–1-AP; 1:1000), AURKA (Cell Signaling Technology, Danvers, MA, USA; 14475T; 1:1000), AURKB (Cell Signaling Technology; 28711T; 1:1000), RhoC (Cell Signaling Technology; 3430T; 1:1000), RRM2 (Cell Signaling Technology; 65939T; 1:2000), PLK1 (Cell Signaling Technology; 4513T; 1:1000), α-N-catenin (Cell Signaling Technology; 2163T; 1:1000), and GAPDH (Abcam, Cambridge, UK; ab125247; 1:10,000) as the loading control.

    Techniques: Expressing, Molecular Weight, Control